ABSTRACT:
High-quality genomic DNA is the primary requisite for the advanced molecular studies of wild date palms. The presence of high concentration of lipids, polysaccharides, polyphenols, protein molecules, secondary metabolites, along with tough fibrous tissues and environmental contaminants hinders downstream processes where genomic DNA has to be used as a sample. Attempts to isolate the wild date palm DNA following the reported protocols are found not to yield DNA in the expected quality and quantity. Compared to conventional and commercially available methods, the developed protocol is simple, economical, and less time-consuming, without the use of hazardous chemicals. The modified protocol yielded high-quality genomic DNA with A260/280 ratios ranging from 1.78 to 1.88, indicating good purity, and A260/230 ratios close to 2.0, suggesting minimal contamination from polysaccharides, polyphenols, RNA and other significant impurities. In addition to the detailing of the modifications made in the CTAB method, this research article discusses the major step-by-step enhancement among the widely followed DNA isolation protocols. Overall, this study contributes a robust methodological advancement that supports the exploration and utilization of under-researched wild date palm genetic resources.
Cite this article:
Binoy Kurian. Development and Optimization of a Rapid and Efficient Genomic DNA Isolation Protocol for Phoenix sylvestris (L.) Roxb. from Roadside Populations of Madhya Pradesh, Central India. Research Journal of Science and Technology. 2026; 18(4):331-8. doi: 10.52711/2349-2988.2026.00046
Cite(Electronic):
Binoy Kurian. Development and Optimization of a Rapid and Efficient Genomic DNA Isolation Protocol for Phoenix sylvestris (L.) Roxb. from Roadside Populations of Madhya Pradesh, Central India. Research Journal of Science and Technology. 2026; 18(4):331-8. doi: 10.52711/2349-2988.2026.00046 Available on: https://www.rjstonline.com/AbstractView.aspx?PID=2026-18-4-2
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