Author(s):
Misbah Sayyad, Archana Magdum, Aaisha Shaikh, Pratiksha Bhadwankar, Pratiksha Chaugule, Ansar Patel
Email(s):
archurajmane@gmail.com
DOI:
Not Available
Address:
Misbah Sayyad, Archana Magdum*, Aaisha Shaikh, Pratiksha Bhadwankar, Pratiksha Chaugule, Ansar Patel
Sant Gajanan Maharaj College of Pharmacy, Mahagaon, Site Chinchewadi 416503, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 4,
Year - 2026
ABSTRACT:
An efficient, exact, correct, and repeatable reverse-phase high-performance liquid chromatography (RP-HPLC) technique has been established and confirmed to determine salicylic acid (SA) and tolnaftate (TOL) in large and pharmaceutical dosage form. Separations were done by chromatography on an Inertsil ODS-3 C18 column (250 x 4.6mm, 5um), with a mobile phase of acetonitrile:0.1 per cent ortho-phosphoric acid (40:60, v/v) flow rate of 1.0 mL/min. Detection was done at 269nm by means of photodiode array (DAD) detector. SA and TOL time of retention were 3.700 and 4.980min, respectively, and the resolution was 5.70. The technique was confirmed according to the ICH Q2(R1) guidelines of system suitability, linearity, LOD, LOQ, and robustness. The correlation coefficients of 0.9999 and 0.9998 between the 16-80ug/mL (SA) and 2-10ug/mL (TOL) established linearity respectively. The LODs were 0.68ug/mL (SA) and 0.17ug/mL (TOL); the LOQs were 2.05ug/mL (SA) and 0.53 ug/mL (TOL). This method was sensitive to the step-wise changes in mobile phase composition (+-1% v/v), and detection wavelength (+-1nm) and in all cases, the resolution was at 5.21 and above. The method developed is easy, not expensive, and can be used during regular quality control of SA and TOL in combined topical pharmaceutical products.
Cite this article:
Misbah Sayyad, Archana Magdum, Aaisha Shaikh, Pratiksha Bhadwankar, Pratiksha Chaugule, Ansar Patel. RP-HPLC Method Development and Validation for Simultaneous Estimation of Salicylic Acid and Tolnaftate in Bulk and Pharmaceutical Dosage Form. Research Journal of Science and Technology. 2026; 18(4):339-5.
Cite(Electronic):
Misbah Sayyad, Archana Magdum, Aaisha Shaikh, Pratiksha Bhadwankar, Pratiksha Chaugule, Ansar Patel. RP-HPLC Method Development and Validation for Simultaneous Estimation of Salicylic Acid and Tolnaftate in Bulk and Pharmaceutical Dosage Form. Research Journal of Science and Technology. 2026; 18(4):339-5. Available on: https://www.rjstonline.com/AbstractView.aspx?PID=2026-18-4-3
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